Blue pippin

Background Bacillus cereus is ubiquitous in nature, found in environments such as soil, plants, air, and part of the insect and human gut microbiome. The ability to produce endospores and biofilms contribute to their pathogenicity, classified in two types of food poisoning: diarrheal and emetic syndromes. Here we report gap-free, whole-genome sequences of two B. cereus strains isolated from ...

Sep 9, 2021 · The Blue Pippin was set to select a size range of 15–50 kb. After collection of the desired fraction, a 1× AMPure XP bead cleanup was performed. The samples were loaded on the PacBio Sequel ... They are all embellished with the same fanciful Early Bird pattern, consisting of a light blue background, adorned with a bird and exotic flowers. The porcelain ...Are you looking to purchase a used RV? If so, you’ve come to the right place. Kelley Blue Book is the go-to source for used vehicle pricing and research, and they have a wealth of information to help you get the most out of your used RV pur...Unsupported-Protocol-Bacterial-Iso_Seq-Clontech-SMARTer-PCR-cDNA-Synthesis-Kit-BluePippin-Size-SelectionSelection Chart Pippin Prep BluePippin PippinHT Max. DNA input 5 μg 5 μg 1.5 μg Max. Sample Capacity 5 5 24 Pulsed-Field No Yes Yes Run Times (100 bp - 1.5 kb) 50-90 min 50-90 min* 25-45 min* Size Selection Range 100 bp - 1.5 kb 100 bp - 50 kb 100 bp - 1.5 kb High-Pass Size Filtering No Yes Yes Low-Pass Size Filtering Yes Accuracy > 93% ...

Page 6 P/N 100-286-000-07 STEP Purify DNA Notes 1 Add 0.45X volume of AMPure PB beads to the End-Repair reaction. (For detailed instructions on AMPure PB bead purification, see the Concentrate DNA section.) 2 Mix the bead/DNA solution thoroughly. 3 Quickly spin down the tube (for 1 second) to collect the beads. Do not pellet beads.The contribution of snoRNAs to the regulation of normal and malignant hematopoiesis is largely unknown. Chu et al reported that overexpression of the H/ACA box snoRNA ACA11 in t(4;14)-associated multiple myeloma contributes to myeloma cell proliferation and resistance to chemotherapy. 11 Several groups have reported marked …The Pippin Prep facilitates library construction for the most popular NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows. The platform features the ability to collect narrow and even fragment distributions, as well as the flexibility to collect wide ranges of fragments with minimal effort.We’ve taken the pulsed-field power supply out of the BluePippin instrument to provide researchers an affordable tool to resolve DNA out to 100 kb and beyond. The Pippin Pulse features a simple software interface to provide users full flexibility to develop their own field-inversion protocols. The operating range is 25-175V, and up to 200 mA ... 13 thg 8, 2022 ... The Blue Tiger. oil on canvas • 40.64 x 71.12 cm. Horace Pippin - February 22, 1888 - July 6, 1946 Horace Pippin ...Pippin depicted everyday events, historical figures, and religious themes using a simplified style of flat shapes and strong colors. In the American popular press of the 1910s and 1920s, the blue tiger was a recurring symbol of the unattainable, and the black bear signified the wilderness. The tiger and bear, rendered in contrasting tones, seem ...

Blue Pippin Cassette 1.5%, 250-1,500 bp, Internal Marker. Gel cassettes for DNA selections on the Blue Pippin. For smaller fragment sizes, internal standards are used for higher accuracy and increased sample capacity. Higher molecular weight fragments require a dedicated lane to run a calibration marker.Services - BluePippin (SageScience) • BluePippin Cassette kits • Online Submission Request • Physical Submission of Samples. The BluePippin (jpg) has the capabilities of the Pippin Prep, plus pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer with no ...The importance of structural variants (SVs) for human phenotypes and diseases is now recognized. Although a variety of SV detection platforms and strategies that vary in sensitivity and specificity have been developed, few benchmarking procedures are available to confidently assess their performances in biological and clinical research.BluePippin Operations Manual 460013 Rev K 1-1 1 Introduction Thank you for purchasing the BluePippin from Sage Science. The BluePippin is anFor the Blue Pippin protocol, the SMRTbell templates were size-selected using the Sage Science BluePippin instrument with a 4 kb or 7 kb lower cutoff, depending on DNA quality. In both protocols, PacBio Sequencing Primer was then annealed to the SMRTbell template library, and sequencing polymerase was bound to them using Sequel Binding Kit 2.0. ...

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Biscotti is a rare indica dominant hybrid strain (80% indica/20% sativa) created through a delicious cross of the classic Gelato #25 X Girl Scout Cookies X South Florida OG strains. If you're looking for a classic indica with an insanely delicious flavor, you've found it. Biscotti packs everything you want and more in the effects and taste ...The BluePippin allows researchers to easily size select and collect from a mixture of DNA fragment sizes and genomic DNA.PippinHT Quick Guide HTC2010 Marker 20B. 1MB. PippinHT Quick Guide HTC1510 Marker 15C. 1MB. PippinHT Quick Guide HPE7510 Marker 75E 6-10Kb and 15-20kb High Pass. 948KB. PippinHT Quick Guide HPF7510 Marker 75F >30-40kb High Pass. 1MB. PippinHT Quick Guide HRT7510 9-30kb Range +T Marker 75E.a, Percentage of the nongap GRCh37 human genome covered by at least ten reads from 28-fold coverage NGS (2 × 250 bp, HiSeq 2500) and CCS (13.5 kb) datasets at different mapping quality thresholds.Seal the PCR plate with foil and centrifuge at 1000 x g for 1 minute at room t emper ature. 5. Place the sealed PCR plate into the therm ocycler. 6. Amplify the cpn60 library following the cpn60 ...

lected on a Sage Science Blue Pippin 1.5% cassette (Sage Science, Beverly, USA) to remove DNA molecules <600bp, and amplification-free, paired end libraries constructed usingBluePippin Operations Manual 460013 Rev D 1-1 1 Introduction Thank you for purchasing the BluePippin from Sage Science. The BluePippin is anBLUE-PIPPIN INSTALLATION PROCEDURE 3.1 Packaging and Components The system is shipped in two boxes: one will contain the BluePippin instrument and accessories, and the second box will contain the computer monitor in the manufacturer's original packaging. With the boxes in the upright position, open and confirm that the following items are ...The synthesized cDNA was enriched by PCR amplification, the amplified cDNA fragments were divided into different size fractions using the Blue Pippin size selection system (Pacific Biosciences).Blue Pippin Size-Selection System. The first step of the protocol was cDNA synthesis with adapters. and PCR cycle optimization followed by large-scale PCR amplification. PCR products were then size.Page 1 PN 101-987-800 Version 02 (November 2021) Procedure & Checklist - Preparing HiFi SMRTbell® Libraries from Ultra-Low DNA Input . This procedure describes preparing HiFi SMRTbell libraries from 5 ng of input genomic DNA (gDNA) forThe larger sample wells now allow a maximum load of 10 μg (100% up from standard Blue Pippin cassettes). Also the size of the elution module has been increased as well as the surface area of the filtration membrane, bringing about improved sample recovery and reproducibility. Blue Pippin software requirement: v6.31/6.40 CD31selected protocol will be highlighted in blue. g. For the target duration, enter the desired run time (typically 14 to 16 hours). Note that the default value is 2 hours. Consider optimizing the run time based on the ambient lab temperature since the Pippin Pulse Electrophoresis System does not regulate the temperature in the gel chamber.lected on a Sage Science Blue Pippin 1.5% cassette (Sage Science, Beverly, USA) to remove DNA molecules <600bp, and amplification-free, paired end libraries constructed using8 AFA-TUBE™ TPX Strip (PN 520292) Resource Type: Product Inserts and User Manuals Download PDFBlue Pippin Cassette 1.5%, 250-1,500 bp, Internal Marker. Gel cassettes for DNA selections on the Blue Pippin. For smaller fragment sizes, internal standards are used for higher accuracy and increased sample capacity. Higher molecular weight fragments require a dedicated lane to run a calibration marker.

The repaired/modified DNA was used for V3 adapter Ligation. The adapter ligated library was treated with Enzyme mix for Nuclease treatment to remove damaged or non-intact SMRTbell templates. The purified library was then size selected using two-step size selection with Blue Pippin (Sage Science) generating 9–13 kb and > 15 kb fractions.

Blue Pippin: Automated DNA Size Selection. Collect narrow and even fragment distributions; Flexibility to collect wide ranges of fragments with minimal effort; Pulsed-field electrophoresis for resolving and collecting high molecular weight DNA ...GeneScan 500 ROX dye Size Standard, each. $2.25 (minimum fee $50) GeneScan 500 LIZ dye Size Standard, each. $2.25 (minimum fee $50) GeneScan 400HD ROX dye Size Standard, each. $2.25 (minimum fee $50) MapMarker X-Rhodamine Labeled 50-1000bp, each. $1.80 (minimum fee $50) GeneScan 1200 LIZ dye Size Standard, each.The extended edition version of when Gandalf talks to Pippin about the forces that Mordor will send against Minas Tirith in the coming battle, and of their c...The Blue Pippin is utilized for DNA size selection (up to 50kb) for NGS. Desired target sizes or ranges of sizes are entered in software and fractions are collected in buffer. Up to 5 samples/gel cassette may be run, with no possibility of cross contamination....The Blue Pippin system . from Sage Science enables targeted size selection. High-pass filtering kits for the Blue Pippin are beneficial for long-read sequencing, as they allow the user to collect all the sample above a specified threshold, enabling analysis of only the fragment length of interest. Size selection therefore makes it possible toDouble digest restriction-site associated sequencing (ddRAD-seq) is a flexible and cost-effective strategy for providing in-depth insights into the genetic architecture of germplasm collections.Pippin Prep DNA Size Selection System Selects 100 bp - 1.5 kb. SageELF Whole Sample Fractionation System DNA (up to 40 kb) or Proteins (up to 500 kDa). Pippin Pulse Electrophoresis Power Supply Resolves DNA up to 400kb on a midi gel box. Applications. Transposase Enzyme Linked Long-Read Sequencing ...Re-pool in equal parts Trout Pool 1 & Trout Pool 2 enough to run in duplicate on the Blue Pippin (a minimum of 40uL per sample). Repeat step 1 for Trout Pool 3 & Trout Pool 4. PulThe reason that Pippin does not expect to find any tobacco after leaving the Shire is because the Shire is where tobacco is cultivated for smoking. No other place in Middle Earth does so, though it is found throughout the lands. ... (Capstan Blue) in two-ounce tins. That bill also carries a short note from Tolkien: "Quite correct, & I apologize ...

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Exasperated by, is more like it. "Fool of a Took" is also a historical and genealogical reference. Tooks were adventurous hobbits: Bullroarer, The Old Took. Gandalf chose Bilbo partly because he was Belladona Took's son. Gandalf took Pippin with him to Gondor for protection after the Palantir incident, and answered many of his questions.Pippin Prep; BluePippin; PippinHT; Pippin Pulse; SageELF; Applications. Transposase Enzyme Linked Long-Read Sequencing; DNA Sequencing; RNA Sequencing; Target Capture; Genotyping; MicroRNA; Cell-Free DNA; Proteomics; Support. Video Guides; TELL-Seq; Pippin Prep; BluePippin; PippinHT; HLS Support; Pippin Pulse; SageELF; Resources. Application ...[PIPPIN] My days are brighter than morning air Evergreen pine and autumn blue But all my days are twice as fair If I could share My days with you My nights are warmer than firecoals Incense and stars and smoke bamboo But nights were warm beyond compare If I could share My nights with you To dance in my dreams To shine when I need the sun With youBluePippin Operations Manual 460013 Rev K 1-1 1 Introduction Thank you for purchasing the BluePippin from Sage Science. The BluePippin is anPippin Hill Farm & Vineyards 2015 Easton Blue; Pippin Hill Farm & Vineyards 2019 Cabernet Sauvignon; Pippin Hill Farm & Vineyards 2019 Chardonnay Reserve; Pippin Hill Farm & Vineyards 2019 Wild Common Reserve; Pippin Hill Farm & Vineyards 2020 Chardonnay; Pippin Hill Farm & Vineyards 2020 Reserve Rosé; Pollak Vineyards 2019 Petit Verdot ...Following ligation of SMRTbell adapters, sheared gDNA was size selected with a 4-kb cutoff using Blue Pippin electrophoresis (Sage Science) to generate a greater fraction of long reads capable of resolving repeat sequencing in the A. xylosoxidans genome. Sequencing was performed using the PacBio RS II platform.Page 6 P/N 100-286-000-07 STEP Purify DNA Notes 1 Add 0.45X volume of AMPure PB beads to the End-Repair reaction. (For detailed instructions on AMPure PB bead purification, see the Concentrate DNA section.) 2 Mix the bead/DNA solution thoroughly. 3 Quickly spin down the tube (for 1 second) to collect the beads. Do not pellet beads.Auntie Mabel is knitting a blue jumper for her sister Eadie when she runs out of wool. Frogs Pippin the dog meets some frogs in her garden, and Auntie Mabel finds some frogspawn.Libraries are run through 10-15 cycles of PCR with Kapa Biosystems Library Amplification kits, followed by further size selection with Blue Pippin Prep (Sage Science). Final library quantification is achieved using the Qubit Fluorometer (for concentration) and the Agilent 2100 Bioanalyzer (for library insert size and length). ….

To get the most from your health insurance, you need to make sure that your see providers who are in the Anthem Blue Cross and Blue Shield network. Here are the steps you need to take to find providers that accept Anthem Blue Cross and Blue...Shearing to 10 kb with Covaris G-tubes (5000 x g for 1 min each way) achieves similar results. Alternatively, for longer reads it is recommended not to shear and perform the ligation protocol as recommended, or include a size selection step with the Blue Pippin (Sage Sciences). Sequencing outcomes for all options are given in table below.Specifications. Concentration. 0.75%. Product Type. Agarose Gel Cassette. Quantity. 10 Pack. Provide Content Correction. Sage Science™ BluePippin™ Dye Free 0.75% Agarose Gel Cassettes are disposable and pre-cast cassettes with agarose and running buffer.Gels were run using the Pippin Pulse pulsed-˚eld electrophoresis power supply (PPI 0200). H. Collecting Fractions 2. Remove samples using a standard 100-200µl pippette. Aspirate slowly to minimize breakage of large DNA fragments. Samples will be in a Tris-TAPS buffer at a volume of approximately 80µl. 4. Remove the cassette and dispose of ...Then the large fragments of DNA were recovered by Blue Pippin automatic nucleic acid fragment recovery system, and repaired. Barcode was added by PCR-free method of EXP-NBD104 kit (Oxford Nanopore Technologies Company). The size of fragments was detected by AATI automatic capillary electrophoresis instrument, and the samples were isomolar mixed ...DIVE INTO INDIGO. Trippin' Blue is a denim research trip that inspires through creative innovation and new production horizons. We know how to generate your brand differentiation …The entire elution was size selected on a 3% agarose cassette using the Blue Pippin system, targeting either 190 or 200 bp using “tight” settings. The 40 μL elution was collected from blue pippin and analyzed using High Sensitivity DNA bioanalyzer. Dilution is usually not necessary for this pool, but think about your expected concentration ...This product was recently added by customer request, and is available for your convenience. We strive to provide our customers with a one-stop shop for the entire scientific supplies category.Background. Orius laevigatus, a minute pirate bug, is a highly effective beneficial predator of crop pests including aphids, spider mites and thrips in integrated pest management (IPM) programmes.No genomic information is currently available for O. laevigatus, as is the case for the majority of beneficial predators which feed on crop pests.In contrast, genomic information for crop pests is far ... Blue pippin, [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1]